Effect of an Antibacterial Monomer on the Antibacterial Activity of a Pit-and-Fissure Sealant
نویسندگان
چکیده
Resin-based pit-and-fissure sealants are often used to form a barrier on the occlusal surface of molars to treat caries lesions; however, bacteria can remain in the pit and fissures without detection, increasing the risk of secondary caries. Sealants with antimicrobial properties or microbial repellent actions might be advantageous. The aim of this study was to assess the inhibitory effect of a 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB)-incorporated sealant against Streptococcus mutans. MAE-DB (4% wt) was incorporated into a commercially available sealant, Eco-S resin-based pit-and-fissure sealant (Vericom Co., Ltd., Korea); a sealant without MAE-DB served as a negative control, and Clinpro™ Sealant (3M™ ESPE™), a fluoride-releasing resin, was used as a commercial control. The effects of the cured sealants and their eluents on the growth of S. mutans were determined according to colony-forming unit counts and metabolic tests. The effects of the cured sealants on the adherence and membrane integrity of S. mutans were investigated using confocal laser-scanning microscopy (CLSM) in conjunction with fluorescent indicators. Compared with the negative control and commercial control, the cured MAE-DB-incorporated pit-and-fissure sealant exhibited a significant inhibitory effect on the growth of S. mutans (P < 0.05), whereas the eluents did not show any detectable antibacterial activity. The commercial control also showed no detectable bactericidal activity. Moreover, the aged experimental material retained its property of contact inhibition of biofilm formation. The fluorescence analysis of CLSM images demonstrated that the cured MAE-DB-incorporated sealant could hamper the adherence of S. mutans and exert a detrimental effect on bacterial membrane integrity. The incorporation of MAE-DB can render a pit-and-fissure sealant with contact antibacterial activity after polymerization via influencing the growth, adherence, and membrane integrity of S. mutans. Therefore, MAE-DB-containing pit-and-fissure sealant shows promise for preventing or controlling dental caries on occlusal pit and fissures of molars.
منابع مشابه
Effect of chlorhexidine solution on microleakage of pit and fissure sealants in permanent teeth with and without the use of fifth- and sixth-generation adhesive systems
Introduction: Due to concerns about histological caries and recurrent caries after the use of sealants, adhesive systems containing fluoride and antibacterial agents have been proposed. The aim of this study was to evaluate the microleakage of sealants after the use of antibacterial chlorhexidine solution on etched enamel with and without the use of fifth- and sixth-generation adhesive systems....
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TITLE Antibacterial properties of fluoride releasing glass Ionomer cements (GICs) and pit and fissure sealants on Streptococcus mutans. BACKGROUND Occlusal pit and fissures are the most susceptible sites for dental caries. The clinical effectiveness of GICs and fissure sealants in preventing caries is well-documented, but there is some concern about bacteria left beneath sealants. OBJECTIVE...
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